LOCUS       40924_39168        4506 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Thermosensitive suicide vector pSET4s DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Takamatsu D, Osaki M, Sekizaki T.
  TITLE     Thermosensitive suicide vectors for gene replacement in 
            Streptococcus suis
  JOURNAL   Plasmid 46 (2), 140-148 (2001)
  PUBMED    11591139
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Takamatsu D, Osaki M, Sekizaki T.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2001) Daisuke Takamatsu, National Institute of 
            Animal Health, Laboratory of Molecular Bacteriology; Kannondai 
            3-1-1, Tsukuba, Ibaraki 305-0856, Japan 
            (E-mail:p1013dt@niah.affrc.go.jp, Tel:81-298-38-7743, 
            Fax:81-298-38-7743)
REFERENCE   3  (bases 1 to 4506)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4506)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2001"; volume: "46"; issue: "2"; pages: "140-148"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-FEB-2001) Daisuke Takamatsu, National Institute of Animal 
            Health, Laboratory of Molecular Bacteriology"; volume: " Kannondai 
            3-1-1, Tsukuba, Ibaraki 305-0856, Japan 
            (E-mail:p1013dt@niah.affrc.go.jp, Tel:81-298-38-7743, Fax"; pages: 
            "81-298-38-7743"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(133..144)
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     CDS             528..689
                     /codon_start=1
                     /gene="orfC"
                     /product="ORFC"
                     /label=orfC
                     /protein_id="BAB64880.1"
                     /translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEYA
                     RKESEQKK"
     gene            528..689
                     /gene="orfC"
                     /label=orfC
     CDS             756..1454
                     /codon_start=1
                     /gene="repAts"
                     /product="RepAts"
                     /label=repAts
                     /protein_id="BAB64881.1"
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNNSNIIQNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     gene            756..1454
                     /gene="repAts"
                     /label=repAts
     primer_bind     2036..2052
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2053..2109
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2122..2138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2146..2162)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2170..2200)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2215..2236)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2524..3112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             3637..4404
                     /codon_start=1
                     /gene="spc"
                     /product="spectinomycin adenyltransferase"
                     /label=spc
                     /protein_id="BAB64883.1"
                     /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS
                     DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ
                     EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR
                     RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI
                     LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL"
     gene            3637..4404
                     /gene="spc"
                     /label=spc
ORIGIN
        1 actagttatc ggcataatcg ttaaaacagg cgttatcgta gcgtaaaagc ccttgagcgt
       61 agcgtggctt tgcagcgaag atgttgtctg ttagattatg aaagccgatg actgaatgaa
      121 ataataagcg cagcgccctt ctatttcggt tggaggaggc tcaagggagt atgagggaat
      181 gaaattccct catgggtttg attttaaaaa ttgcttgcaa ttttgccgag cggtagcgct
      241 ggaaaatttt tgaaaaaaat ttggaatttg gaaaaaaatg gggggaaagg aagcgaattt
      301 tgcttccgta ctacgacccc ccattaagtg ccgagtgcca atttttgtgc caaaaacgct
      361 ctatcccaac tggctcaagg gtttaagggg tttttcaatc gccaacgaat cgccaacgtt
      421 ttcgccaacg ttttttataa atctatattt aagtagcttt attgttgttt ttatgattac
      481 aaagtgatac actaacttta taaaattatt tgattggagt tttttaaatg gtgatttcag
      541 aatcgaaaaa aagagttatg atttctctga caaaagagca agataaaaaa ttaacagata
      601 tggcgaaaca aaaaggtttt tcaaaatctg cggttgcggc gttagctata gaagaatatg
      661 caagaaagga atcagaacaa aaaaaataag cgaaagctcg cgtttttaga aggatacgag
      721 ttttcgctac ttgtttttga taaggtaatt atatcatggc tattaaaaat actaaagcta
      781 gaaattttgg atttttatta tatcctgact caattcctaa tgattggaaa gaaaaattag
      841 agagtttggg cgtatctatg gctgtcagtc ctttacacga tatggacgaa aaaaaagata
      901 aagatacatg gaataatagt aatattatac aaaatggaaa gcactataaa aaaccacact
      961 atcacgttat atatattgca cgaaatcctg taacaataga aagcgttagg aacaagatta
     1021 agcgaaaatt ggggaatagt tcagttgctc atgttgagat acttgattat atcaaaggtt
     1081 catatgaata tttgactcat gaatcaaagg acgctattgc taagaataaa catatatacg
     1141 acaaaaaaga tattttgaac attaatgatt ttgatattga ccgctatata acacttgatg
     1201 aaagccaaaa aagagaattg aagaatttac ttttagatat agtggatgac tataatttgg
     1261 taaatacaaa agatttaatg gcttttattc gccttagggg agcggagttt ggaattttaa
     1321 atacgaatga tgtaaaagat attgtttcaa caaactctag cgcctttaga ttatggtttg
     1381 agggcaatta tcagtgtgga tatagagcaa gttatgcaaa ggttcttgat gctgaaacgg
     1441 gggaaataaa atgacaaaca aagaaaaaga gttatttgct gaaaatgagg aattaaaaaa
     1501 agaaattaag gacttaaaag agcgtattga aagatacaga gaaatggaag ttgaattaag
     1561 tacaacaata gatttattga gaggagggat tattgaataa ataaaagccc cctgacgaaa
     1621 gtcgaagggg gtttttattt tggtttgatg ttgcgattaa tagatctcgg tgatgacggt
     1681 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     1741 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     1801 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     1861 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     1921 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     1981 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     2041 acgacggcca gtgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca
     2101 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     2161 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     2221 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     2281 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2341 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2401 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2461 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2521 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2581 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2641 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2701 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2761 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2821 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2881 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2941 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     3001 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3061 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3121 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3181 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3241 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3301 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3361 cgtgtagata actagtgttc gtgaatacat gttataataa ctataactaa taacgtaacg
     3421 tgactggcaa gagatatttt taaaacaatg aataggttta cacttacttt agttttatgg
     3481 aaatgaaaga tcatatcata tataatctag aataaaatta actaaaataa ttattatcta
     3541 gataaaaaat ttagaagcca atgaaatcta taaataaact aaattaagtt tatttaatta
     3601 acaactatgg atataaaata ggtactaatc aaaatagtga ggaggatata tttgaataca
     3661 tacgaacaaa ttaataaagt gaaaaaaata cttcggaaac atttaaaaaa taaccttatt
     3721 ggtacttaca tgtttggatc aggagttgag agtggactaa aaccaaatag tgatcttgac
     3781 tttttagtcg tcgtatctga accattgaca gatcaaagta aagaaatact tatacaaaaa
     3841 attagaccta tttcaaaaaa aataggagat aaaagcaact tacgatatat tgaattaaca
     3901 attattattc agcaagaaat ggtaccgtgg aatcatcctc ccaaacaaga atttatttat
     3961 ggagaatggt tacaagagct ttatgaacaa ggatacattc ctcagaagga attaaattca
     4021 gatttaacca taatgcttta ccaagcaaaa cgaaaaaata aaagaatata cggaaattat
     4081 gacttagagg aattactacc tgatattcca ttttctgatg tgagaagagc cattatggat
     4141 tcgtcagagg aattaataga taattatcag gatgatgaaa ccaactctat attaacttta
     4201 tgccgtatga ttttaactat ggacacgggt aaaatcatac caaaagatat tgcgggaaat
     4261 gcagtggctg aatcttctcc attagaacat agggagagaa ttttgttagc agttcgtagt
     4321 tatcttggag agaatattga atggactaat gaaaatgtaa atttaactat aaactattta
     4381 aataacagat taaaaaaatt ataaaaaaat tgaaaaaatg gtggaaacac ttttttcaat
     4441 ttttttgttt tattatttaa tatttgggaa atattcattc taattggtaa tcagatttta
     4501 gaaaac
//
